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05-591 Anti-Akt/PKB Antibody, PH Domain, clone SKB1

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05-591
100 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RFC, ICC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-591
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Akt/PKB Antibody, PH Domain, clone SKB1
Background InformationAkt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
PresentationProtein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.
Quality LevelMQ100
Applications
ApplicationDetect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1 validated for use in FC, IC, IP & WB.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenGST-fusion protein corresponding to residues 1-149 of human Akt1
CloneSKB1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes Akt1/PKBα.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Gene Symbol
  • AKT1
  • RAC
  • PRKBA
  • MGC99656
  • RAC-ALPHA
  • RAC-PK-alpha
  • C-AKT
  • PKB
  • AKT
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-591 04053252372124

Documentation

Anti-Akt/PKB Antibody, PH Domain, clone SKB1 SDS

Title

Safety Data Sheet (SDS) 

Anti-Akt/PKB Antibody, PH Domain, clone SKB1 Certificates of Analysis

TitleLot Number
Anti-Akt/PKB, PH Domain, clone SKB1 - 2123701 2123701
Anti-Akt/PKB, PH Domain, -2557589 2557589
Anti-Akt/PKB, PH Domain, -2603798 2603798
Anti-Akt/PKB, PH Domain, -2626023 2626023
Anti-Akt/PKB, PH Domain, -2654216 2654216
Anti-Akt/PKB, PH Domain, -2697425 2697425
Anti-Akt/PKB, PH Domain, -2759752 2759752
Anti-Akt/PKB, PH Domain, -2798557 2798557
Anti-Akt/PKB, PH Domain, clone SKB1 3068460
Anti-Akt/PKB, PH Domain, clone SKB1 (mouse ascites) - DAM1475050 DAM1475050

References

Reference overviewApplicationPub Med ID
Cardiomyocyte-specific BMAL1 plays critical roles in metabolism, signaling, and maintenance of contractile function of the heart.
Young, ME; Brewer, RA; Peliciari-Garcia, RA; Collins, HE; He, L; Birky, TL; Peden, BW; Thompson, EG; Ammons, BJ; Bray, MS; Chatham, JC; Wende, AR; Yang, Q; Chow, CW; Martino, TA; Gamble, KL
Journal of biological rhythms  29  257-76  2014

Show Abstract
Western Blotting25238855 25238855
Resveratrol helps recovery from fatty liver and protects against hepatocellular carcinoma induced by hepatitis B virus X protein in a mouse model.
Lin, HC; Chen, YF; Hsu, WH; Yang, CW; Kao, CH; Tsai, TF
Cancer prevention research (Philadelphia, Pa.)  5  952-62  2012

Show Abstract
22659145 22659145
Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer.
Jones, LW; Antonelli, J; Masko, EM; Broadwater, G; Lascola, CD; Fels, D; Dewhirst, MW; Dyck, JR; Nagendran, J; Flores, CT; Betof, AS; Nelson, ER; Pollak, M; Dash, RC; Young, ME; Freedland, SJ
Journal of applied physiology (Bethesda, Md. : 1985)  113  263-72  2012

Show Abstract
Western Blotting22604887 22604887
Interrogating signaling nodes involved in cellular transformations using kinase activity probes.
Stains, CI; Tedford, NC; Walkup, TC; Luković, E; Goguen, BN; Griffith, LG; Lauffenburger, DA; Imperiali, B
Chemistry & biology  19  210-7  2012

Show Abstract
22365604 22365604
Delayed treatment with a novel neurotrophic compound reduces behavioral deficits in rabbit ischemic stroke.
Lapchak, PA; Schubert, DR; Maher, PA
Journal of neurochemistry  116  122-31  2011

Show Abstract Full Text Article
21054387 21054387
Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo.
Pulinilkunnil, T; He, H; Kong, D; Asakura, K; Peroni, OD; Lee, A; Kahn, BB
The Journal of biological chemistry  286  8798-809  2011

Show Abstract
21209093 21209093
Thermogenic activation induces FGF21 expression and release in brown adipose tissue.
Hondares E, Iglesias R, Giralt A, Gonzalez FJ, Giralt M, Mampel T, Villarroya F
J Biol Chem  2011

Show Abstract
21317437 21317437
Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle.
Treebak, JT; Taylor, EB; Witczak, CA; An, D; Toyoda, T; Koh, HJ; Xie, J; Feener, EP; Wojtaszewski, JF; Hirshman, MF; Goodyear, LJ
American journal of physiology. Cell physiology  298  C377-85  2010

Show Abstract Full Text Article
Western Blotting19923418 19923418
Therapeutically targeting ErbB3: a key node in ligand-induced activation of the ErbB receptor-PI3K axis.
Birgit Schoeberl,Emily A Pace,Jonathan B Fitzgerald,Brian D Harms,Lihui Xu,Lin Nie,Bryan Linggi,Ashish Kalra,Violette Paragas,Raghida Bukhalid,Viara Grantcharova,Neeraj Kohli,Kip A West,Magdalena Leszczyniecka,Michael J Feldhaus,Arthur J Kudla,Ulrik B Nielsen
Science signaling  2  2009

Show Abstract
19567914 19567914
Increased AS160 phosphorylation, but not TBC1D1 phosphorylation, with increased postexercise insulin sensitivity in rat skeletal muscle.
Funai, K; Schweitzer, GG; Sharma, N; Kanzaki, M; Cartee, GD
American journal of physiology. Endocrinology and metabolism  297  E242-51  2009

Show Abstract Full Text Article
19435856 19435856

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Alternative Format

Catalog Number Description
16-293 Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor® 488

Included Positive Control

Catalog Number Description
12-301 Non-Stimulated A431 Cell Lysate

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies