Millipore Sigma Vibrant Logo

05-665-AF555 Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 555

100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
Request Pricing
Fulfilment and delivery delayed
Fulfilment and delivery delayed
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers


      Contact Customer Service


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IHCMAlexaFluor®555Monoclonal Antibody
      Catalogue Number05-665-AF555
      DescriptionAnti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 555
      Alternate Names
      • Catenin beta-1
      • Beta-catenin
      • Active-β-Catenin
      Background InformationCatenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.
      Product Information
      PresentationPurified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      ApplicationThis Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 555 is validated for use in Immunocytochemistry, Immunohistochemistry for the detection of Active-β-Catenin.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in NIH/3T3 cells.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Active-β-Catenin in human colon and human colorectal adenocarcinoma tissue.
      The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).
      Biological Information
      ImmunogenFusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.
      EpitopeThe epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityRecognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Zebrafish based on 100% sequence homology. The antibody eptiope is 100% conserved in zebrafish. .
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTNNB1
      • CTNNB
      • PRO2286
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~85 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 555 SDS


      Safety Data Sheet (SDS) 

      Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 555 Certificates of Analysis

      TitleLot Number
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 555 3063808
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 555 2855644
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 555 2907164
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 555 - 3160115 3160115
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 555 - 3680009 3680009
      Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 555 -Q2592807 Q2592807

      Technical Info

      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

      Related Products & Applications

      Related Products

      Catalog Number Description  
      04-958 Anti-Beta Catenin Antibody, clone 7F7.2 Show Pricing & Availability
      05-613 Anti-β-Catenin Antibody, clone 2H4A7 Show Pricing & Availability
      05-665 Anti-Active-β-Catenin (Anti-ABC) Antibody, clone 8E7 Show Pricing & Availability
      05-665-AF488 Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 Show Pricing & Availability
      05-665-AF647 Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 647 Show Pricing & Availability
      06-734 Anti-β-Catenin Antibody Show Pricing & Availability
      ABE208 Anti-β-Catenin Antibody Show Pricing & Availability
      MAB2081 Anti-β-Catenin Antibody, clone 5H10 Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies