Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
This Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 is validated for use in Immunocytochemistry, Flow Cytometry, Immunohistochemistry for the detection of Active-β-Catenin.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in A431 cells. Flow Cytometry Analysis: 0.1 µg of this antibody from a representative lot detected Active-β-Catenin in 1X10E6 A431 and HeLa cells. Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Active-β-Catenin in human colon and human colorectal adenocarcinoma tissue. The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).
Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.
The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
Please refer to lot specific datasheet.
Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
Species Reactivity Note
Human, Mouse. Predicted to react with Rat, Zebrafish based on 100% sequence homology. The antibody eptiope is 100% conserved in zebrafish. .
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.
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