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05-665-AF488 Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488

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05-665-AF488
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, FC, IHCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-665-AF488
DescriptionAnti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488
Alternate Names
  • Catenin beta-1
  • Beta-catenin
  • Active-β-Catenin
Background InformationCatenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 is validated for use in Immunocytochemistry, Flow Cytometry, Immunohistochemistry for the detection of Active-β-Catenin.
Key Applications
  • Immunocytochemistry
  • Flow Cytometry
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in A431 cells.
Flow Cytometry Analysis: 0.1 µg of this antibody from a representative lot detected Active-β-Catenin in 1X10E6 A431 and HeLa cells.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Active-β-Catenin in human colon and human colorectal adenocarcinoma tissue.
The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).
Biological Information
ImmunogenFusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.
EpitopeThe epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.
Clone8E7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityRecognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Zebrafish based on 100% sequence homology. The antibody eptiope is 100% conserved in zebrafish. .
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CTNNB1
  • CTNNB
  • PRO2286
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~85 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-665-AF488 04055977275155

Documentation

Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 SDS

Title

Safety Data Sheet (SDS) 

Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor® 488 Certificates of Analysis

TitleLot Number
Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 488 3083597
Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 488 2959461
Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 488 2822004
Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 488 - 3227305 3227305
Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 488 - 3291385 3291385
Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 488 - 4000736 4000736
Anti-Active-β-Catenin, clone 8E7, Alexa Fluor® 488 -Q2592803 Q2592803

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies