ST1673 Anti-AFP Mouse mAb (1G7)

ST1673
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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ST1673-100UG
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      Glass bottle 100 μg
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      Description
      OverviewRecognizes the ~70-75 kDa AFP protein in HepG2 cells.
      Catalogue NumberST1673
      Brand Family Calbiochem®
      SynonymsAnti-α-Fetoprotein
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHepG2 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
      Clone1G7
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-AFP Mouse mAb (1G7) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-AFP Mouse mAb (1G7) Certificates of Analysis

      TitleLot Number
      ST1673
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-α-Fetoprotein
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
      BackgroundThis gene encodes α-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. α-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of α-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the α-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. α-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of α-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
      Clone1G7
      IsotypeIgG
      Specieshuman
      Positive controlHepG2 cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review