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MAB312R Anti-A2B5 Antibody, clone A2B5-105

MAB312R
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MaFC, ICC, IF, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB312R
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-A2B5 Antibody, clone A2B5-105
      Alternate Names
      • Neuron Cell Surface Antigen
      Background InformationA2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors and neuroendocrine cells. The A2B5 antigen is present in type 2 astrocytes but absent in type 1 astrocytes, allowing for the differentiation of these different types of cells. A2B5 antibody has been used in flow cytometry and immunohistochemistry experiments to stain type II astrocytes, cells involved in gliogenesis and cells committed to the oligodendrocyte lineage.
      References
      Product Information
      FormatPurified
      Control
      • Type II astrocytes, human neural progenitors
      PresentationLiquid. Buffer = PBS, pH 7.1 with 2 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-A2B5 Antibody, clone A2B5-105 detects level of A2B5 & has been published & validated for use in FC, IC, IF, IH.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunocytochemistry: 5-10 μg/mL Immunohistochemistry: 5-10 μg/mL (Levison & McCarthy, 1989)

      Complement-mediated cytotoxicity (Eisenbarth et al., 1979)

      Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenEmbryonic chicken retinal cells
      CloneA2B5-105
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:

      Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer's disease (Majocha et al., 1989)
      Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979).
      Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989)].
      Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).
      Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).
      Human and rat thymic epithelial cells (Haynes et al., 1988).
      Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & Slerberg, 1989).
      Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).
      IsotypeIgM
      Species Reactivity
      • Mammals
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • A2B5
      Purification MethodAmmonium Sulfate Precipitation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain the lyophilizate frozen at -20°C. Store the reconstituted antibody solution at -20°C for 12 months in undiluted aliquots.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-A2B5 Antibody, clone A2B5-105 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-A2B5 Antibody, clone A2B5-105 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-A2B5 - 3179203 3179203
      MOUSE ANTI-A2B5 - 3596791 3596791
      MOUSE ANTI-A2B5 - 3840868 3840868
      MOUSE ANTI-A2B5 - 3889506 3889506
      MOUSE ANTI-A2B5 -2819627 2819627
      MOUSE ANTI-A2B5 MONOCLONAL ANTIBODY 3046755
      MOUSE ANTI-A2B5 MONOCLONAL ANTIBODY - 2393922 2393922
      MOUSE ANTI-A2B5 MONOCLONAL ANTIBODY - 2000813 2000813
      MOUSE ANTI-A2B5 MONOCLONAL ANTIBODY - 2020429 2020429
      MOUSE ANTI-A2B5 MONOCLONAL ANTIBODY - 2092682 2092682

      Data Sheet

      Title
      MOUSE ANTI-A2B5

      Related Products & Applications

      Alternative Format

      Catalog Number Description  
      MAB312 Anti-A2B5 Antibody, clone A2B5-105 Show Pricing & Availability
      MAB312RX Anti-Neuron Cell Surface Antigen Antibody, clone A2B5-105 (7), Alexa Fluor® 488 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies