Product Information
Applications
Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.
Key Applications Affinity Purification
Immunoblotting (Western Blotting)
Application NotesAffinity Purification (see application references)
Immunoprecipitation (15 µl beads/sample)
Immunoprecipitation (1-2 µg/ml; see application references)
Immunocytochemistry (use Cat. No. NA02)
Paraffin Sections (use Cat. No. NA02)
Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
Biological Information
Immunogen2,2,7-trimethylguanosine
CloneK121
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate Certificates of Analysis

TitleLot Number
NA02A

References

Reference overview
Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
Kramer, A., et al. 1984. Cell 38, 299.
Lerner, M.R., et al. 1980. Nature 283, 220.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
ApplicationAffinity Purification (see application references)
Immunoprecipitation (15 µl beads/sample)
Immunoprecipitation (1-2 µg/ml; see application references)
Immunocytochemistry (use Cat. No. NA02)
Paraffin Sections (use Cat. No. NA02)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Conjugated to agarose. Recognizes the exposed 5' cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
BackgroundThe U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
HostMouse
Immunogen2,2,7-trimethylguanosine
CloneK121
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlHeterogenous nuclear RNA (hnRNA)
Negative controlTransfer RNA (tRNA)
FormLiquid suspension
FormulationIn PBS.
Preservative≤0.1% sodium azide
CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
Kramer, A., et al. 1984. Cell 38, 299.
Lerner, M.R., et al. 1980. Nature 283, 220.
Application referencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.

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Life Science Research > Antibodies and Assays > Primary Antibodies