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96-Well Plasmid and BAC Preparation Kit

Simply clear, concentrate, wash, and recover

Overview

Specifications

Ordering Information

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96-Well Plasmid and BAC Preparation KitClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack Size
LSKP09604Plasmid Miniprep96 Kit 4 Show Pricing & Availability
LSKP09624Plasmid Miniprep96 Kit 24 Show Pricing & Availability

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AccessoriesClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack Size
SCGVU01REStericup-GV Sterile Vacuum Filtration System 12 Show Pricing & Availability

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Required EquimentClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack Size
MSVMHTS00MultiScreenHTS Vacuum Manifold 1 Show Pricing & Availability
WP6111560Chemical Duty Pump, 115 V/60 Hz 1 Show Pricing & Availability
WP6122050Chemical Duty Pump, 220 V/50 Hz 1 Show Pricing & Availability
WP6110060Chemical Duty Pump, 100 V/50–60 Hz 1 Show Pricing & Availability

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Reagents*Clear Sorting & Filtering Show Filter
Catalog NumberDescriptionVolume
LSKCRS500Cell Resuspension Solution 0.5 L 500 mL Show Pricing & Availability
LSKCLS500Cell Lysis Solution 0.5 L 500 mL Show Pricing & Availability
LSKNS0500Neutralization Solution, 0.5 L 500 mL Show Pricing & Availability
LSKNF0500Nuclease-free Water, 0.5 L 500 mL Show Pricing & Availability
LSKCTB500Tris Buffer, 0.5 L 500 mL Show Pricing & Availability
LSKPMRN30RNase A, 0.9 mL 0.9 mL Show Pricing & Availability

*All reagents are supplied 1 bottle/pack

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Documentation

FAQ

QuestionAnswer
Can I use this kit in centrifugal mode?Use of the Montage Plasmid Miniprep-96 Kit in a centrifugal protocol is not recommended.
I have observed RNA contamination of my plasmid preps. Why?Failure to add RNase A to solution 1 will often result in RNA contamination in your DNA miniprep.
I have observed that the wells of the MultiScreen plate used in the Montage kit are not filtering uniformly. Why?The wells of the MultiScreen plates usually filter uniformly. However, a bubble in the lysate may cause an airlock, which halts filtration. Also, a highly viscous sample may increase filtration time considerably.
When using the Montage kits how can I tell when filtration in the MultiScreen plate is complete?Filtration is complete when the individual wells of the MultiScreen plate are empty and the membranes appear shiny.
What is holdup volume of a MultiScreen Plate used with the Montage Kits?The holdup volume for the MultiScreen plate is between 5-20 microliters.
The two sections of the MultiScreen plates used in the Montage kits are separating under vacuum. Why?The most common reason for this problem is the lack of a MultiScreen underdrain support grid in the vacuum manifold. A MultiScreen support grid is required for proper functioning of the MultiScreen plate with any vacuum manifold.
What is the total processing time for plasmid preparation using the Montage kits?Processing time depends on viscosity of sample, number of manifolds, familiarity with protocol, temperature. (Also, need vacuum capable of 24 in Hg or 812.7 millibar, 609.6 torr.)
Can the culture block in the Montage 96 Plasmid prep be autoclaved?Yes.
The total yields with the Montage Plasmid Miniprep-96 kit seems to be less then with competitive products. Why?The Montage kit uses less culture volume (1ml vs. 1.3 ml) than most competitive kits. However the relative yield is equivalent.
What is the 260/280 assessment ratio when using the Montage Plasmid Kit?The 260/280 reading is 1.8-2.0. This signifies and extremely high level of purity.