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506047 3,3ʹ-Diamino-4ʹ-methoxyflavone, DD1 - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-amino-2-(3-amino-4-methoxyphenyl)-4H-chromen-4-one, p76S6 Inhibitor, DD1, Proteasome Inhibitor, DD1

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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₆H₁₄N₂O₃
Description
OverviewA cell-permeable flavone derivative that arrests the proliferation of acute myeloid leukemia cells (NB4, U937, HL-60, OCI AML3), in a dose and time-dependent manner (optimum at ~20 µM and 96 h) and blocks the colony formation in samples from acute myeloid leukemia patients, without affecting normal blood cells. Shown to reduce mitochondrial membrane potential and Bad phosphorylation (at Ser136), induce Bax and caspase 3, 8, and 9 activation in U937 cells. Does not affect the phosphorylation of Akt1, but significantly reduces the expression and phosphorylation of p70S6 kinase in U937 cells. Reported to inhibit the chymotrypsin-like activity of proteasome (IC50 = 2.0 µM) and this effect is synergistic with bortezomib (Cat. No. 504314).
Catalogue Number506047
Brand Family Calbiochem®
Synonyms3-amino-2-(3-amino-4-methoxyphenyl)-4H-chromen-4-one, p76S6 Inhibitor, DD1, Proteasome Inhibitor, DD1
References
ReferencesPiedfer. M., et al, 2013. Biochim. Biophys. Acta. 1833,1316
Quiney, C., et al, 2004. Leuk. Res. 28, 851.
Product Information
FormYellow powder
Hill FormulaC₁₆H₁₄N₂O₃
Chemical formulaC₁₆H₁₄N₂O₃
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ200
Applications
Biological Information
Primary Target IC<sub>50</sub>2 µ
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Identification Number
Catalog Number GTIN
506047

Documentation

3,3ʹ-Diamino-4ʹ-methoxyflavone, DD1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Piedfer. M., et al, 2013. Biochim. Biophys. Acta. 1833,1316
Quiney, C., et al, 2004. Leuk. Res. 28, 851.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-December-2013 JSW
Synonyms3-amino-2-(3-amino-4-methoxyphenyl)-4H-chromen-4-one, p76S6 Inhibitor, DD1, Proteasome Inhibitor, DD1
DescriptionA cell-permeable flavone derivative that arrests the proliferation of acute myeloid leukemia cells (NB4, U937, HL-60, OCI AML3), in a dose and time-dependent manner (optimum at ~20 µM and 96 h) and blocks the colony formation in samples from acute myeloid leukemia patients, without affecting normal blood cells. Shown to reduce mitochondrial membrane potential and Bad phosphorylation (at Ser136), induce Bax and caspase 3, 8, and 9 activation in U937 cells. Does not affect the phosphorylation of Akt1, but significantly reduces the expression and phosphorylation of p70S6 kinase in U937 cells. Reported to inhibit the chymotrypsin-like activity of proteasome (IC50 = 2.0 µM) and this effect is synergistic with bortezomib (Cat. No. 504314).
FormYellow powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₆H₁₄N₂O₃
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesPiedfer. M., et al, 2013. Biochim. Biophys. Acta. 1833,1316
Quiney, C., et al, 2004. Leuk. Res. 28, 851.