The ProteoExtract® Glycopeptide Enrichment Kit is designed to capture high-mannose, hybrid, and complex type glycosylated peptides from complex protein mixtures.
ProteoExtract® All-In-One Trypsin Digestion Kit is optimized for the digestion of proteins by trypsin either in-gel, in-solution or in-suspension. Digested samples are suitable for analysis on mass spectroscopy, HPLC, or electrophoresis.
The ProteoExtract® Phosphopeptide TiO2 Enrichment Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by a highly selective Titanium oxide solid phase.
The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC).
Improved digestion of hard-to-digest proteins
Ovalbumin and ovalbumin digests were analyzed as follows: lane 1: ovalbumin, chicken egg, 43 kDa; lane 2: digest incubated 3 h using ProteoExtract® All-in-One Trypsin Digestion Kit; lane 3: digest incubated 4 h using the same kit; lane 4: digest incubated 3 h using reagent from Supplier I; lane 5: digest incubated 3 h using kit from Supplier II.
Titanium dioxide allows for selective and sensitive phosphopeptide enrichment with low background from complex mixtures.
Enhanced detection allows comprehensive analysis
Ovalbumin was digested using ProteoExtract® All-in-One Trypsin Digestion Kit (panel A) or according to Supplier III kit protocol (panel B). Base peak chromatograms of nano-LC/MS analyses are shown.