Millipore Sigma Vibrant Logo

Single Molecule Counting (SMC™) Immunoassay Technology

Request Information

Related Resources: Brochure | Analyte Quarterly

Merck:/Freestyle/BI-Bioscience/Protein-Detection/bmia-images/AN-1804-20-Hamilton-thumb.jpgLearn how automating your SMC™ assays with the Hamilton Microlab® STARlet can help improve your efficiency and precision

Download Now

New Video!
Learn how Single Molecule Counting (SMC™) technology works using the SMCxPRO™ Instrument


Watch Now

New Poster
Merck:/Freestyle/BI-Bioscience/Protein-Detection/singulex/PS3845EN_MS-thumb.jpgDiscover how IL-17A and IL-17F ultrasensitive assays were evaluated using human endogenous biomarkers and human plasma matrix

Download Now

Watch Our New Webinar
Getting it right. The importance of rigorous validation for high-sensitivity immunoassays.

Watch Now

Expert Panel Discussion
What does the future hold for biomarker R&D?

Register Now

Download Your Copy of the New Analyte Quarterly
Analyte QuarterlyFind the perfect set of assays!

Get Your Copy

Introducing the SMCxPRO™ System

In the News
Accelerating Drug Development & Clinical Validation with Single Molecule Counting

Read DD&D Article

Unleashing the Sensitivity of Single Molecule Counting (SMC™) Technology

Ultrasensitive SMC™ technology, originally developed by Singulex®, Inc., provides an indispensable tool in the researcher’s arsenal to help them move novel biology forward, fueling the discovery and development of new therapeutics.

MilliporeSigma now offers the power of SMC™ technology to leading pharmaceutical R&D laboratories, clinical research organizations (CROs) and academic institutions around the world through the Research-Use-Only SMCxPRO™ and Erenna® immunoassay systems, immunoassay kits, reagents, and custom services.

SMCxPRO™ Instrument
SMCxPRO™ Instrument with SMC™ technology quantifies low-abundance biomarkers, on the bench top.

Previously Undetectable, Now Quantified. One Molecule at a Time.

SMC™ Single Molecule Counting (SMC™) technology enables precise measurement of molecules at levels previously undetectable, for unprecedented biomarker insights.

Scientists familiar with traditional immunoassays, such as ELISAs, know that sensitivity, matrix effects, and dynamic range affect how they make measurements — or if they can make them at all.

Combining a traditional immunoassay workflow with patented SMC™ technology enables the detection of low-abundance biomarkers, such as proteins and nucleic acids, with unparalleled sensitivity and accuracy, capturing concentrations down to the femtogram/mL level. Researchers can now detect, and monitor changes in, extremely low levels of established disease biomarkers such as cardiac troponin I and cytokines.


What Can Better Precision and Sensitivity Do for Your Research?

  • Quantify previously undetectable analytes
  • Better stratify sample populations
  • Gain insights into novel biological mechanisms
  • Require fewer data points for critical decision making
  • Accelerate drug discovery and development
  • Reduce program costs and improve productivity

SMC™ Assay Workflow. During the capture and detection steps, specific antibodies translate each biomarker into signal. During the modified elution step, fluorescent dye-labeled detection antibodies are dissociated from the immunocomplex. The eluate is measured in a small interrogation space illuminated by a laser. Single fluorescently labeled molecules are excited by the laser as they pass through the interrogation space as detected events.

The SMCxPRO™ and Erenna® instruments capture the sum of all digital events counted above a background threshold. This model can have limited dynamic range so at higher concentrations on the Erenna® a proprietary algorithm computes the total sum of all photons recorded as an additional standard curve and the SMCxPRO™ digital event algorithm computes digital events across a spectrum of time series for a single standard curve thus improving assay sensitivity and extending the dynamic measuring range beyond what can be achieved with traditional technologies.