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Media and Cell Culture Reagents

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Featured Product:
Embryomax® ES Qualified Fetal Bovine Serum (US Origin)
Merck:/Freestyle/BI-Bioscience/Cell-Culture/cell-culture-images/fetal-bovine-serum.jpgThe maintenance, expansion, and assay of many stem cell and precursor cell cultures relies on media rich in a variety of factors, often supplied by fetal bovine serum (FBS). Each lot of such sera must be carefully controlled and tested to ensure proper stem cell growth.
Cell culture – especially stem cell culture –requires high quality media and reagents. To ensure reproducible results, media must be pure, free of contaminants, and consistent from lot to lot. To support your stem cell research, MilliporeSigma offers a wide range of high quality media, specialty buffers, growth factors, and other reagents. Popular items include our Embryomax® line of media, Accutase™ and Accumax™ cell dissociation and detachment solutions, a wide range of extracellular matrix proteins and growth factors, and more.

Classic Media

Featured Product:
Accutase™ and Accumax™
- Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
- The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting.

Freezing Media

MilliporeSigma’s unique cell culture freezing media are ideal for the cryopreservation of a broad spectrum of mammalian cells. With both DMSO and glycerol formulations, these products result in consistent cryopreservation and high cell viability upon thawing and plating. These sterile reagents are virus- and mycoplasma-free and come with a complete protocol that has been successfully used on the following cell lines:

129SvEv C6 FRTL MOLT-4 PC-12
293 CCE GH3 MRC-5 Psi-2
A10 CEM HEL NC-37 Raji
AfT-20 CHO HeLa NIH 3T3 Rat2
BHK-21 D-17 HT-29 OKT4 U-937
BRK Daudi HUT78 OKT8 Vero
C127I E-14 L Cells P-19 WI-38
C3H10T1/2 EB1 LLC-MK P815 WISH
C57/BL6 F9 MDCK PA317  

Dissociation Reagents

Enzyme Free Dissociation Solutions-Our unique non-enzymatic solutions, which contain no protein or surfactants, are composed of chelating agents and other agents used to stabilize their activity on the cells. These reagents gently dislodge adherent cells from their substrates, while preserving the structural and functional integrity of cell surface proteins. There are no cytotoxic effects associated with these solutions, such as those sometimes associated with the use of 0.5 mM EDTA. They can be used to dissociate primary cells, tissues, and tumors, while allowing for increased efficiency.