Rhodamine B in Chili Extract


Safe Detection Using Purospher® HPLC Columns

Rhodamine B in chili extract

Red chili extract (red paprika oleoresin) is highly stable in light and heat, and is soluble in oil, acetone, chloroform, n-hexane and alcohol. After emulsification, the extract can be transformed into a water-soluble or water-separate product. Sudan dyes, which belong to the class of synthetic azo dyes, have been previously added to bell pepper and chili powders in order to intensify their color. However, their metabolites are considered to be both carcinogens and teratogens. Consequently, the EU and US have banned the use of these colors as food additives. Nevertheless, these dyes are still occasionally used in some countries. Accurate analysis techniques are necessary in order to avoid potential health risks. MilliporeSigma's Purospher® HPLC columns are ideal for the detection of Rhodamine B in chili extract. The columns offer highly reliable results, with excellent separations and outstanding peak symmetry.

Application Example

Separation of Rhodamine B in chili extract

Column Purospher® STAR RP-18 endcapped (5μm) LiChroCART® 250-4,6 HPLC column (150252)
Precolumn Purospher® STAR RP-18 endcapped (5μm) LiChroCART® 4-4 (150250)
Oven temperature 40 °C
Injection volume 10 μL
Flow Rate 1 mL /min
Eluent A Phosphoric acid (1 mL 85% Phosphoric acid in to 1 L of Water)
Eluent B Acetonitrile
GradientTime (min)Eluent AEluent BFlow Rate (mL/min)
0.0
0.0
15.0
20.0
21.0
35.0
58
58
20
20
58
58
42
42
80
80
42
42
1
1
1
1
1
1
Detection Fluorescence
Wavelength Wavelength
PMT Voltage Medium
Sample Rate 400 ms
Time Constant 1 sec
Sample 0.6248 g Oleoresin oil in 5 mL eluent (Oleoresin sample contaminated with 140 ppb Rhodamine B)

Specification of Purospher® RP-18 endcapped

Sorbent characteristics Purity C18-silica with special modification and deactivation of the surface
Metal content Na, Ca, Mg, Al: 1 ppm; Fe 3 ppm
Particle shape Spherical
Particle size 5 μm
Pore size 90 Å (9 nm)
Pore volume 1.05 mL/g
Spec. surface area 480 m²/g
Carbon load 18.0 % C
pH range pH 2 - 8
Efficiency 80.000 N/m
Shipping eluent Acetonitrile/Water
 
Purospher® HPLC columns

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