Process chemicals for biochromatography

Buffers and salts - For the sake of your protein

The purpose of a downstream buffer is to create a defined environment for maintaining biomolecules in a stable native form while retaining their full activity. The advantage here is the minimized risk, so come to us and play it safe, from start to finish.

Broad selection to choose from

As a large buffer manufacturer, EMD offers a wide range of organic and inorganic buffers and buffer salts, specifically tested for use in downstream purification.

Protect your protein

Recent additions to the range include buffer salts such as phosphates, acetates or citrates in the quality grade EMPROVE® bio. Manufacturing processes have been optimized by minimizing microbiological contamination and endotoxin concentrations – enzymatic activities of DNases, RNases and proteases are not detectable. This is what makes these buffers particularly suitable for biochromatography. Your protein is so valuable that it should not be put at risk so shortly before the end of manufacture.

Please do not hesitate to contact us for more information on EMPROVE® or for the full product catalog.

Additives

For cell disruption, our portfolio includes disruptive agents such as Toluol, Triton X-100, and Lysozyme.

Also available: our virus inactivation and refolding agents tripeptide glutathione (reduced), caprylic acid and Gluthathione, all of pharmacopoeial quality.

Enzymes for DNA removal

Unique and most efficient product: Benzonase® endonuclease

Removal of DNA and RNA brings an overall improvement in further downstream processing-steps. The best method for this is enzymatic cleavage by means of Benzonase®, a non-specific, recombinant endonuclease produced from E.coli and which separates DNA and RNA into fragments comprising <10 soluble base pairs, in keeping with regulatory criteria stipulating that fragments need to be <100 bp.

You want to eliminate nucleic acids from recombinant proteins?

Benzonase® endonuclease is the only effective biochemical method of removing DNA and RNA in the laboratory and in industrial-scale processes. This product is a unique, genetically engineered endonuclease, not of animal origin, offering a variety of advantages over existing methods of removal.

High purity

Our product ensures that you will meet the highest possible purity standards for biopharmaceuticals. Benzonase® endonuclease is manufactured and exclusively distributed by Merck KGaA, Darmstadt, Germany, and its subsidiaries. The production techniques used to manufacture Benzonase® endonuclease ensure a product of exceptionally high quality and purity.

Ideal for a variety of applications

Benzonase® endonuclease is free of detectable proteolytic activity and possesses an exceptionally high specific activity. It is effective over a wide range of operating conditions and is ideal for a variety of applications including purification of proteins and other biologicals, reduction of viscosity caused by nucleic acids, and sample preparation in electrophoresis and chromatography.

Reduce process time

It has proven its value in the laboratory and is successfully applied in various processes throughout the pharmaceutical and biotechnological industry.

Maximize productivity

You can maximize your productivity thanks to the higher purity, higher recovery and easier scale-up.

Would you like further information? Please contact us.