BPHCVD05  WideScreen^® BeadPlex^® Human CVD Panel 5

EMD Millipore is focusing our development and operational efforts on our in-house MILLIPLEX MAP assays. This line of products offers over 700 different analytes, in multiple research areas, species, and both circulating and intracellular biomarkers.

This product has been discontinued. Please use the following MILLIPLEX MAP kit for your future studies: MILLIPLEX MAP Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 (Cat. No. HCVD3MAG-67K).

xMAP^® technologies is a multiplex bead-based assay platform that allows for sensitive, precise quantification of multiple analytes within a single sample. For the detection of proteins or peptides, the assay kits often utilize the familiar sandwich or competitive immunoassay formats (e.g. ELISA). Unlike ELISA though, the target analytes are captured on differentially fluorescently-dyed beads. This differential allows for the detection of multiple analytes from the same sample in the same well. The BeadPlex^® assays are run by combining the sample(s) with the capture antibody-coated beads. The specific analytes from the sample are captured on the beads and are then subsequently identified with detection antibodies and a fluorescent label using an appropriate instrument. A major advantage over traditional protein quantification methods (such as ELISA) is the capacity for multiplexing, as bead-based assays allow simultaneous quantification of multiple analytes in a small sample volume.

Panel Overview

Conditions that affect the heart or vasculature are termed cardiovascular diseases (CVD) and include hypertension, congenital heart disease, and coronary heart disease. As a group, CVDs are a heterogeneous set of conditions and there are numerous causative and accessory factors. Atherosclerosis (narrowing and hardening of the arteries), internal bleeding and blood clots, inflammation, infection, genetic and environmental factors underlie many CVDs and are the subject of large and growing research and therapeutic efforts.

Acute phase proteins (also known as acute phase reactants) are characterized as proteins for which levels increase or decrease as a consequence of inflammatory conditions. Complement proteins, coagulation proteins, protease inhibitors, metal binding proteins, and others are induced as part of the acute phase response (APR). An APR is not localized to the site of inflammation, but rather is a systemic, multi-organ response that normally lasts from hours to days. Conditions that can induce an APR include infection, tissue trauma or death, burns, and cancer. Levels of several acute phase proteins are also elevated in cardiovascular disease.

The WideScreen BeadPlex Human CVD Panel 5 is designed to detect up to eight human cardiovascular disease proteins found in serum, plasma, and tissue culture supernatants. The CVD Panel 5 includes three types of immunoassays. CRP, Fetuin A, Lp(a), SAP, and vWF are conventional sandwich assays. Fibrinogen is a competitive assay where antibody capture of a biotinylated antigen competes for analyte binding. Alpha 2-macroglobulin and haptoglobulin assays are competitive assays where antigen is immobilized to the bead and binds biotinylated detection antibody in the blocking buffer; upon sample addition, analyte can compete away bound detection antibody, resulting in a decrease in fluorescent signal. See the following table for a list of analytes detectable by the BeadPlex Human CVD Panel 5 and the respective bead regions.

This novel multi-analyte profiling kit provides a deeper view of the following antigens and proteins in normal and disease processes:

• Alpha 2-Macroglobulin (α2-M)
• C-reactive protein (CRP)
• Fetuin-A
• Fibrinogen
• Haptoglobin
• Lipoprotein(a) (Lp(a))
• Serum Amyloid P (SAP)
• von Willebrand Factor (vWF)

Panel Components

Storage: All components of the BeadPlex Human CVD Panel 5 are shipped at 2 to 8°C. Upon receipt, store all kit components at 2 to 8°C. Do Not Freeze.

The BeadPlex Human CVD Panel 5 is available in two kit configurations:

• Complete Kit
• Mix and Match Format.

For utmost convenience, end-users may order the BeadPlex Human CVD Panel 5 - Complete Kit (Cat. No. BPHCVD05-8). This kit contains all respective capture beads and all the components in the BeadPlex Human CVD Panel 5 - Base Kit. Desired capture beads will need to be pre-mixed prior to running the assay. A Complete Kit contains enough components to assay one 96-well plate.

For maximum flexibility, end-users may configure their own customized multiplex assay of any or all specified analytes through the Mix and Match Format. This format consists of a BeadPlex Human CVD Panel 5 - Base Kit (Cat. No. BPHCVD05-BASE) and a customized choice of any or all analyte-specific capture beads as listed in Technical Bulletin (TB544). The Base Kit and selected components contain enough reagents and buffers to assay one 96-well plate.