BPHCP002  WideScreen^® BeadPlex^® Human Cancer Panel 2

EMD Millipore is focusing our development and operational efforts on our in-house MILLIPLEX MAP assays. This line of products offers over 700 different analytes, in multiple research areas, species, and both circulating and intracellular biomarkers.

This product has been discontinued. Please use the following MILLIPLEX MAP kits for your future studies: MILLIPLEX MAP Human Angiogenesis/Growth Factor Magnetic Bead Panel (Cat. No. HAGP1MAG-12K), MILLIPLEX MAP Human Soluble Cytokine Receptor Panel (Cat. No. HSCR-32K), and MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Cat. No. HCYTOMAG-60K) (contains the majority of the analytes).

xMAP^® technologies is multiplex bead-based assay platform that allows for sensitive, precise quantification of multiple analytes within a single sample. For the detection of proteins or peptides, the assay kits often utilize the familiar sandwich or competitive immunoassay formats (e.g. ELISA). Unlike ELISA though, the target analytes are captured on differentially fluorescently-dyed beads. This differential allows for the detection of multiple analytes from the same sample in the same well. The BeadPlex^® assays are run by combining the sample(s) with the capture antibody-coated beads. The specific analytes from the sample are captured on the beads and are then subsequently identified with detection antibodies and a fluorescent label using an appropriate instrument. A major advantage over traditional protein quantification methods (such as ELISA) is the capacity for multiplexing, as bead-based assays allow simultaneous quantification of multiple analytes in a small sample volume.

Panel Overview

Many growth factors are thought to play a role in cancer progression. Levels of EGFR and related growth factors are often elevated or dysregulated in tumor cells and can be detected in blood samples from cancer patients. EGFR activation is initiated through the autocrine or paracrine binding of EGF and EGF-like growth factors to the extracellular domain of EGFR or other members of the ErbB family, resulting in receptor dimerization and initiation of signal transduction cascades. Amphiregulin, EGF, TGF-a, betacellulin, epiregulin, and HB-EGF all bind to EGFR, and in some cases ErbB-4. Normal and malignant cells also produce soluble forms of EGFR through alternate mRNA splicing or proteolytic cleavage of the transmembrane receptor. Many therapeutic approaches targeting EGFR have been developed; however, combinational therapy directed at cancer-related growth factors is still an emerging area of research that may prove effective in overcoming tumor resistance.

Tumor cells induce new blood vessel formation by secreting a variety of growth factors that act on endothelial cells. VEGF-A is the major stimulator of angiogenesis; nevertheless, the coordination of multiple cytokines is required including: EGF, FGF-basic, PDGF-BB, PlGF, Tenascin C, and TGF-a. In turn, angiogenesis promotes tumor metastasis, providing a means for malignant cells to establish at distal sites. Cancerous tumors frequently express elevated levels of angiogenic growth factors, and therefore can be useful as diagnostic and prognostic markers. For instance, tenascin C expression correlates with angiogenesis and carcinoma metastasis.

This novel multi-analyte profiling kit provides a deeper view of the following individual growth factors and soluble EGFR receptor levels in normal and disease processes:

• Amphiregulin (AREG)
• Betacellulin (BTC)
• Epidermal Growth Factor (EGF)
• Epidermal Growth Factor Receptor (EGFR)
• Epiregulin (EREG)
• Fibroblast Growth Factor-basic (FGF-basic, FGF-2)
• Heparin-Binding Epidermal Growth Factor (HB-EGF)
• Platelet-Derived Growth Factor-BB (PDGF-BB)
• Placenta Growth Factor (PlGF)
• Tenascin C (TnC)
• Transforming Growth Factor-alpha (TGF-α)
• Vascular Endothelial Growth Factor-alpha (VEGF-A)

Panel Components

Storage: All components of the BeadPlex Human Cancer Panel 2 (Growth Factors) are shipped at 2 to 8°C. Upon receipt, store all kit components at 2 to 8°C. Do not freeze.

The BeadPlex Human Cancer Panel 2 is available in two kit configurations:
• Complete Kit
• Mix and Match Format.

For utmost convenience, end-users may order the BeadPlex Human Cancer Panel 2 - Complete Kit (Cat. No. BPHCP002-12). This kit contains all respective capture beads and all components in the BeadPlex Human Cancer Panel 2 - Base Kit. Desired capture beads will need to be pre-mixed prior to running the assay. The Complete Kit contains enough components to assay one 96-well plate.

For maximum flexibility, end-users may configure their own customized multiplex assay through the Mix and Match Format. This format consists of a BeadPlex Human Cancer Panel 2 - Base Kit (Cat. No. BPHCP002-BASE) and a customized choice of any or all analyte-specific capture beads as listed in Technical Bulletin (TB538). The Base and selected components contain enough reagents and buffers to assay one 96-well plate.