BPHCP001  WideScreen^® BeadPlex^® Human Cancer Panel 1

EMD Millipore is focusing our development and operational efforts on our in-house MILLIPLEX MAP assays. This line of products offers over 700 different analytes, in multiple research areas, species, and both circulating and intracellular biomarkers.

This product has been discontinued. Please use the following MILLIPLEX MAP kit for your future studies: MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel (Cat. No. HCCBP1MAG-58K) (contains all of the analytes).

xMAP^® technologies is multiplex bead-based assay platform that allows for sensitive, precise quantification of multiple analytes within a single sample. For the detection of proteins or peptides, the assay kits often utilize the familiar sandwich or competitive immunoassay formats (e.g. ELISA). Unlike ELISA though, the target analytes are captured on differentially fluorescently-dyed beads. This differential allows for the detection of multiple analytes from the same sample in the same well. The BeadPlex™ assays are run by combining the sample(s) with the capture antibody-coated beads. The specific analytes from the sample are captured on the beads and are then subsequently identified with detection antibodies and a fluorescent label using an appropriate instrument. A major advantage over traditional protein quantification methods (such as ELISA) is the capacity for multiplexing, as bead-based assays allow simultaneous quantification of multiple analytes in a small sample volume.

Panel Overview

Tumor markers selected for the BeadPlex Human Cancer Panel 1 are known to be directly or indirectly involved in various types of cancers. a-fetoprotein is a major fetal plasma protein. It passes into the maternal bloodstream where it may be detected by the assay. Elevated AFP is observed most frequently in patients with germ cell tumors and hepatocellular carcinoma, but also in patients with gastric, colon, biliary, pancreatic and lung cancers. Cancer antigen 125 is a glycoprotein found in increased amounts on the cell surface of many ovarian epithelial malignancies. Elevated levels of CA 125 in the blood are associated with serous, endometrioid, and clear cell carcinomas of the ovary. Malignancies of breast, lung, and gastrointestinal (GI) tract may also be associated with an elevation of this tumor marker. Cancer antigen 15-3 is predominantly associated with late-stage breast cancers. Levels are seldom elevated in patients with early-stage breast cancer. Patients with cancers of the ovary, lung, or prostate may also have elevated CA 15-3 levels. Cancer antigen 19-9 is a derivative of the Lea blood group antigen present on normal mesenteric epithelium. It is expressed at high levels in most patients with carcinomas of the pancreas, liver, biliary tract, stomach, colon, and breast.

Carcinoembryonic antigen is observed in extracts of colonic carcinoma tissue. Elevated levels are found in serum from patients with a wide variety of tumor types including GI, lung, breast, ovary, and uterus. Prolactin is a peptide hormone primarily associated with lactation. It is responsible for stimulating breast tissue growth and differentiation under normal circumstances; however, it can also stimulate the growth of breast cancers that may arise.

This novel multi-analyte profiling kit provides a deeper view of the following antigens and proteins in normal and disease processes:

• alpha-fetoprotein (AFP)
• Cancer antigen 125 (CA 125)
• Cancer antigen 15-3 (CA 15-3)
• Cancer antigen 19-9 (CA 19-9)
• Carcinoembryonic antigen (CEA)
• Prolactin

Panel Components

Storage: All components of the BeadPlex Human Cancer Panel 1 are shipped at 2 to 8°C. Upon receipt, store all kit components at 2 to 8°C. Do not freeze.

The BeadPlex Human Cancer Panel 1 is available in two kit configurations:

• Complete Kit
• Mix and Match Format.

For utmost convenience, end-users may order the BeadPlex Human Cancer Panel 1 - Complete Kit (Cat. No. BPHCP001-6). This kit contains all respective capture beads and all the components in the BeadPlex Human Cancer Panel 1 - Base Kit. Desired capture beads will need to be pre-mixed prior to running the assay. The kit contains enough components to assay one 96-well plate.

For maximum flexibility, end-users may configure their own customized multiplex assay of 1-6 specified analytes through the Mix and Match Format. This format consists of a BeadPlex Human Cancer Panel 1 - Base Kit (Cat. No. BPHCP001-BASE) and a customized choice of any or all analyte-specific capture beads as listed in Technical Bulletin (TB537). The Base Kit and selected components contain enough reagents and buffers to assay one 96-well plate.