Information
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pRSFDuet-1 Vector Sequence

LOCUS       pRSFDuet-1   3829 bp    DNA   circular            29-JUL-2003
DEFINITION  pRSFDuet-1/Kan.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|304553949|
COMMENT     VNTDBDATE|309451894|
COMMENT     VNTNAME|pRSFDuet-1|
COMMENT     VNTAUTHORNAME|EMD Biosciences Inc.|
COMMENT     VNTAUTHORTEL|608-442-4000|
COMMENT     VNTAUTHOREML|novagen@emdbiosciences.com|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     CDS             complement(739..1554)
                     /vntifkey="4"
                     /label=Kan
     CDS             complement(2607..3689)
                     /vntifkey="4"
                     /label=lacI
                     /note="Wildtype lacI gene"
     promoter        3813..3829
                     /vntifkey="30"
                     /label=T7\Promoter
     protein_bind    5..25
                     /vntifkey="31"
                     /label=lacO
     RBS             58..63
                     /vntifkey="32"
                     /label=RBS-1
     misc_binding    83..100
                     /vntifkey="20"
                     /label=His-Tag
     primer          complement(448..466)
                     /vntifkey="27"
                     /label=T7\Terminator\primer
     terminator      462..509
                     /vntifkey="43"
                     /label=T7\Terminator
     protein_bind    366..410
                     /vntifkey="31"
                     /label=S-Tag
     CDS             300..428
                     /vntifkey="4"
                     /label=MCS-2\ORF
     protein_bind    235..255
                     /vntifkey="31"
                     /label=lacO
     promoter        214..230
                     /vntifkey="30"
                     /label=T7\promoter
     RBS             286..291
                     /vntifkey="32"
                     /label=RBS
     misc_RNA        complement(1662..2360)
                     /vntifkey="53"
                     /label=RNA\II
     misc_RNA        2251..2357
                     /vntifkey="53"
                     /label=RNA\I
     rep_origin      1662..2411
                     /vntifkey="33"
                     /label=RSF\ori\-\RSF1030\Homology
     -10_signal      2239..2244
                     /vntifkey="47"
                     /label=-10\RNA\I
     -35_signal      2215..2220
                     /vntifkey="48"
                     /label=-35\RNA\I
     RBS             complement(1560..1564)
                     /vntifkey="32"
                     /label=RBS
     -35_signal      complement(1619..1624)
                     /vntifkey="48"
                     /label=-35\Ap
     -10_signal      complement(1596..1602)
                     /vntifkey="47"
                     /label=-10\Ap
BASE COUNT      979 a      1003 c       907 g       940 t 
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag 
      121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag 
      181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt 
      241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata 
      301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt 
      361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg 
      421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg 
      481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg 
      541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt 
      601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt 
      661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
      721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt 
      781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa 
      841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg 
      901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa 
      961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca 
     1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc 
     1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca 
     1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt 
     1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt 
     1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat 
     1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc 
     1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt 
     1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat 
     1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt 
     1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     1621 tgaatgtatt tagaaaaata aacaaatagg catgcagcgc tcttccgctt cctcgctcac 
     1681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaagcggt 
     1741 aatacggtta tccacagaat caggggataa agccggaaag aacatgtgag caaaaagcaa 
     1801 agcaccggaa gaagccaacg ccgcaggcgt ttttccatag gctccgcccc cctgacgagc 
     1861 atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc 
     1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt 
     2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     2161 acgacttatc gccactggca gcagccattg gtaactgatt tagaggactt tgtcttgaag 
     2221 ttatgcacct gttaaggcta aactgaaaga acagattttg gtgagtgcgg tcctccaacc 
     2281 cacttacctt ggttcaaaga gttggtagct cagcgaacct tgagaaaacc accgttggta 
     2341 gcggtggttt ttctttattt atgagatgat gaatcaatcg gtctatcaag tcaacgaaca 
     2401 gctattccgt tactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc 
     2461 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg 
     2521 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga 
     2581 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc 
     2701 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg 
     2761 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt 
     2821 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact 
     2881 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc 
     2941 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc 
     3001 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga 
     3061 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa 
     3121 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg 
     3181 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag 
     3241 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg 
     3301 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc 
     3361 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc 
     3421 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga 
     3481 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg 
     3541 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa 
     3601 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct 
     3661 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg 
     3721 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg 
     3781 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata 
//

© Merck KGaA, Darmstadt, Germany, 2014

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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