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71090  UltraMobius™ 200 Plasmid Kit

“Midi” scale, ultra-low endotoxin plasmid DNA for the most demanding applications

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26 May 2012

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The UltraMobius™ 200 Plasmid Kit is designed for fast, convenient, and economical isolation of > 200 µg ultra-low endotoxin plasmid DNA from 35-ml overnight bacterial cultures, using high-copy number plasmid (> 30 µg for low-copy number plasmids). The kit features the same high-capacity anion exchange tentacle resin as the other Mobius™ kits and incorporates a unique filter basket that minimizes the centrifugation steps required for clarification of bacterial lysates. The UltraMobius 200 Kit includes a Detox™ Agent to reduce endotoxin levels below 20 EU/mg plasmid DNA. Purifiied plasmid DNA is suitable for the most demanding applications such as DNA microinjections and transfection of sensitive cell lines.


Product information
Components
25Mobius 200 Columns
25Mobius Filters
1 mlRNase A
30 mlDetox™ Agent
100 mlBacterial Resuspension Buffer
100 mlBacterial Lysis Buffer
100 mlMobius Neutralization Buffer
125 mlMobius Equilibration Buffer
250 mlMobius Wash Buffer
65 mlMobius Elution Buffer
12.5 mlTE Buffer
Store and ship information
Storage +15°C to +30°C
Ship Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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