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70906  UltraMobius™ 1000 Plasmid Kit

“Maxi” scale, ultra-low endotoxin plasmid DNA for the most demanding applications

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26 May 2012

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70906-3  10 rxn  This Product is currently discontinued or not available.
70906-4  25 rxn 
This Product is currently discontinued or not available.
 
Prices are subject to change without notice.

The UltraMobius 1000 Plasmid Kits is designed for fast, convenient, and economical isolation of up to 1 mg ultra-low endotoxin plasmid DNA from 100 ml overnight bacterial culture, when isolating high-copy number plasmids (Hendriks 2000). For low-copy number plasmid, the kit can isolate up to 200 µg DNA from a 250-ml culture and up to 1 mg from 500-ml to 1.5-L cultures using a scalable protocol. The kits features the same high-capacity anion exchange tentacle resin as the other Mobius™ kits and incorporates a novel ClearSpin™ filtration device that minimizes the duration of centrifugation steps required for the clarification of lysates. The UltraMobius 1000 Kits include a Detox™ Agent to reduce endotoxin levels to below 20 EU/mg plasmid DNA. Purified plasmid DNA is suitable for the most demanding applications such as DNA microinjections and transfection of sensitive cell lines.


Product information
Components
10 rxn25 rxn
1025Mobius 1000 Columns
1025ClearSpin Filters
22ClearSpin Adapters
1 ml2 × 1 mlRNase A
100 ml2 × 100 mlBacterial Resuspension Buffer
100 ml2 × 100 mlBacterial Lysis Buffer
100 ml2 × 100 mlMobius Neutralization Buffer
30 ml2 × 30 mlDetox Agent
125 ml2 × 125 mlMobius Equilibration Buffer
250 ml2 × 250 mlMobius Wash Buffer
65 ml2 × 65 mlMobius Elution Buffer
12.5 ml2 × 12.5 mlTE Buffer
Store and ship information
Storage +15°C to +30°C
Ship Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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