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EMD Millipore
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26 May 2012
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This product has been discontinued.
We apologize for the inconvenience, but we do not currently have an alternative product.
For related products, please refer to our complete listing of kinase ELISA kits.
Detects and quantifies the levels of c-Src protein, independent of its phosphorylation state. c-Src is the member of homologous non-receptor tyrosine kinases family with two major phosphorylation sites on Src at Tyr426 and Tyr527. Activation of c-Src is critically involved in carcinoma cell adhesion, migration, metastasis and the enhancement of cell survival. Although this kit is designed for human cell lines, platelets and lymphocytes, it cross-reacts with mouse and rat cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Sensitivity | ≤1 ng/ml | ||
| Assay range | 1.6 - 50 ng/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates | ||
| Kit contains | Src Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 The analytical sensitivity of this assay is <1 ng/ml of Src (Total). This was determined by adding two standard deviations to the mean Abs obtained when the zero standard was assayed 30 times. The sensitivity of this ELISA was compared to immunoblotting using known quantities of Src. The data presented in Figure 1 show that the ELISA is at least as sensitive as immunoblotting. The bands shown in the immunoblotting data were developed using rabbit anti-Src, an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent substrate and autoradiography. |
