69282
Single dA™ Tailing Kit
Addition of single 3′-dA overhangs for cloning into AccepTor™ Vectors
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
loading
The Single dA™ Tailing Kit is used to prepare
DNA with any type of end for cloning into the
Novagen AccepTor™ Vectors. The kit provides
the reagents needed to blunt DNA ends and
add a single 3′-dA residue. This treatment
creates vector-compatible overhangs and
effectively prevents insert-to-insert ligation,
thus eliminating tandem inserts. The positive
control included in the kit is the blunt-ended
HSV•Tag® 36-mer that encodes the
11-aa peptide recognized by the HSV•Tag
Monoclonal Antibody.
Applications
- Treatment of PCR products of proofreading DNA polymerases for cloning into AccepTor Vectors (alternative to blunt-end cloning)
- Treatment of randomly cut or sheared DNA to be cloned into dU- or dT-tailed vectors
| Product information | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Avoid freeze/thaw | Yes | |||||||||||||||||||||||
| Components |
|
|||||||||||||||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |
||
