Rosetta™(DE3)pLysS Competent Cells
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01821 Billerica, MA
10 March 2014
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE (CamR)
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Dry Ice Only
Multiple Toxicity Values, refer to MSDS
|Licence Brookhaven||This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.|