71255  RoboPop™ Solubility Screening Kit

Expression and solubility screening in a 96-well format

This product has been discontinued.

The RoboPop™ Solubility Screening Kit is designed for protein expression-level and solubility screening in a 96-well format and includes all reagents and plates necessary to perform parallel processing of 96 bacterial cultures. The kit contains PopCulture® Reagent and Lysonase™ Bioprocessing Reagent for efficient extraction of recombinant proteins from E. coli directly from the culture medium without cell harvest, mechanical disruption, or extract clarification. Lysonase significantly increases protein extraction efficiency and reduces sample viscosity facilitating downstream processing and robotic pipetting.



The RoboPop™ Solubility Screening Kit also incorporates an innovative filtration plate capable of retaining insoluble inclusion bodies while allowing soluble proteins to be collected for rapid quantitation and analysis. Insoluble proteins retained by the filtration plate are solubilized with 4% SDS, collected, and quantified separately. The Filter Plate is compatible with standard vacuum manifolds for manual processing and the entire protocol has been validated for robotic sample processing with the Genesis® Freedom™ Workstation from Tecan and the MultiPROBE® II liquid handling work station from PerkinElmer Life Sciences. Two collection plates with sealers are provided for convenient storage of separated soluble and insoluble fractions.