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EMD Millipore
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26 May 2012
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The ProteoExtract® Phosphopeptide TiO2 Enrichment Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by a highly selective Titanium oxide solid phase. It enhances the ability to routinely identify and characterize large numbers of phosphorylated species within complex protein mixtures taking advantage of a novel titanium dioxide material. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis.
| Product information | |||
|---|---|---|---|
| Format | Phosphopeptide enrichment | ||
| Form | 100 Isolations | ||
| Detection method | Mass Spectrometry | ||
| Kit contains | TiO2 Phosphobind Resin, 10X TiO2 Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, TiO2 Elution Buffer, Dihydroxybenzoic Acid, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 <b>Titanium dioxide allows for selective and sensitive phosphopeptide enrichment with low background from complex mixtures.</b> A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides (see below for concentrations). The samples were subsequently processed using the ProteoExtract<sup>®</sup> Phosphopeptide Enrichment TiO<sub>2</sub> Kit as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode. (A) Unprocessed sample (B) Phosphopeptides recovered using the ProteoExtract<sup>®</sup> Phosphopeptide Enrichment TiO<sub>2</sub> Kit. <b>Spiked Phosphopeptides:</b> 1. 50 nM Angiotensin ~6 pmol/125 µl; ~7 ng/125 µl 2. 67 nM Calcineurin Substrate ~8 pmol/125 µl; ~18 ng/125 µl 3. 140 nM α-Casein ~18 pmol/125 µl; ~400 ng/125 µl |
