539486
Protein Kinase A, Catalytic Subunit, Bovine Heart
cAMP-Dependent Protein Kinase Catalytic Subunit
Convenient kit for site-specific 32P-labeling of target proteins
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
26 May 2012
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The PKAce™ Kit is designed for in vitro
labeling of proteins in a simple reaction
with 32P-γ-ATP and purified Protein Kinase
A (PKA). Proteins can be labeled to at least
105 cpm/µg in 10 minutes, and can be used
directly as sensitive and specific probes for
protein:protein interactions (1). The PKAce
Kit includes a highly purified preparation of
bovine heart PKA, optimized reaction buffer,
and PKA Control Protein for verification of kit
performance.
Mediates the majority of the actions of cAMP. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Stimulates Ca2+-dependent exocytosis in bovine chromaffin cells. Suitable for studies of PKA phosphorylation in vitro. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
| Substitutes | |
|---|---|
| 539576 | PKA, Catalytic Subunit, Bovine Heart |
| Substitutes for Protein Kinase A, Catalytic Subunit, Bovine Heart | |
| 539576 | PKA, Catalytic Subunit, Bovine Heart |
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM NaCl, 30 mM DTT, 20 mM MES, 100 µM EDTA, 50% glycerol, pH 6.5. | ||
| Molar mass | 40,000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥750 units/µg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to histone per min at 30°C. | ||
| Purity | ≥95% by SDS-PAGE | ||
| EC number | 2.7.1.37 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | DEEP FREEZE (-70°C) | ||
| Ship |
Dry Ice Only
Standard Handling |
||
