PopCulture® His•Mag™ Purification Kit
PopCulture® extraction and His•Mag purification in a 96-well format
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01821 Billerica, MA
Phone: 781 533-6000
21 May 2013
The PopCulture® His•Mag™ Purification Kit is designed for purification of His•Tag® fusion proteins directly from E. coli cultures without harvesting cells. The procedure combines PopCulture total culture extraction with magnetic affinity purification using His•Mag Agarose Beads.
PopCulture Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for centrifugation. Use of His•Mag Agarose Beads enables the entire procedure to be carried out in a single tube without using columns or centrifugation.
The PopCulture His•Mag Purification Kit combines PopCulture Reagent, His•Mag Agarose Beads, corresponding buffers, and rLysozyme™ Solution. This kit enables the processing of 40 × 3-ml cultures with yields up to 375 µg His•Tag® fusion protein per 3-ml culture, based on bead binding capacity. The kit is compatible with the Novagen Magnetight™ Separation Stand. For 96-well processing using PopCulture, try the RoboPop™ His•Mag Purification Kit.
|Features and benefits||
|Declaration||This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.|
|Store and ship information|
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
|Storage||Multiple storage requirements|
Blue Ice Only
Multiple Toxicity Values, refer to MSDS