539482
PKA, His•Tag®, Active, Human, Recombinant, E. coli
Protein Kinase A, cAMP Dependent Protein Kinase
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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recombinant, human, catalytic subunit of PKA fused to at the N-terminus to a His•Tag® sequence and expressed in E.coli. PKA regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for labeling PKA substrates.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 150 mM KCl, 30 mM K2HPO4, 1 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.4. | ||
| Molar mass | 42301 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥ 500,000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to PKA peptide (GRTGRRNSI) per min at 30°C. | ||
| Purity | ≥95% by SDS PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Increasing amounts of PKA were subjected to SDS-PAGE and purity analyzed by Coomassie Blue staining.  Increasing amounts of PKA were analyzed for activity as outlined in the protocol above. |
