539481
PKA, Catalytic Subunit, Mouse, Recombinant, E. coli
cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit
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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
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E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5. | ||
| Molar mass | 38000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥5 x 106 U/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5. | ||
| Activity | ≥2.5 x 106 U/ml | ||
| Contaminants | Phosphatases, proteases: none detected | ||
| EC number | 2.7.1.37 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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