539576
PKA, Catalytic Subunit, Bovine Heart
cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
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EMD Millipore
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25 May 2012
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Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
Note: 25 µg ≥ 500,000 units.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 350 mM K2HPO4, 100 µM DTT, pH 6.8. | ||
| Molar mass | 40000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥20,000 units/µg protein | ||
| Unit definition | One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C. | ||
| Purity | ≥95% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
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