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69091  pIE1-4 DNA

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25 May 2012

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The pIE1-4 vector is designed for constitutive expression of recombinant proteins from the baculovirus ie1 promoter in stably-transformed insect cells (1). The plasmid contains all promoter sequences known to be important for ie1-mediated gene expression in uninfected insect cells as well as the hr5 enhancer element. pIE1-4 lacks an ie1-driven translation initiation site; therefore, the ATG start codon must be supplied by the insert. To obtain stably-transformed cells, a pIE1-4 recombinant is cotransfected with pIE1-neo, which contains the Neo selectable marker under ie1 control.
Product information
Avoid freeze/thaw Yes
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Storage FREEZER (-20°C)
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© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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