Phosphodiesterase, Type II, Calf Spleen
For general questions please contact our Customer Service:
290 Concord Road
01821 Billerica, MA
Phone: 781 533-6000
26 May 2013
This product has been discontinued.
We apologize for the inconvenience, but we do not currently have an alternative product.
For related products please refer to our complete listing of phosphodiesterases.
Native phosphodiesterase type II from calf spleen. Useful in sequence studies of oligonucleotides derived from RNA and DNA. Attacks the 5′-terminal end of phosphodiester bonds, releasing 3′-mononucleotides.
|Formulation||In 3.2 M amounium sulfate, 50 mM ammonium acetate, 50 mM NaCl, 0.1% Tween®-80 Detergent, pH 6.0.|
|Do not freeze||Yes|
|Specific activity||≥7 units/mg protein|
|Unit definition||One unit is defined as the amount of enzyme that will produce 1 µmol of 3′-thymidine-phosphate per min at 25°C after hydrolysis of thymidylyl(3′→5′)thymidine (TpT). Dilute in deionized water just before use.|
|Contaminants||Phosphomonoesterase, 3'-phosphodiesterase: none detected|
|pH value||optimum 6.0|
|Store and ship information|
|Storage||+2°C to +8°C|
Blue Ice Only