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524746  Phosphodiesterase 4D5, Active, Human, Recombinant, S. frugiperda

cAMP-specific phosphodiesterase 4D isoform 5, PDE4D5

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23 May 2013

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Full-length, recombinant, human phosphodiesterdase 4D5 (PDE4D5) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE4D5 is expressed primarily in cardiac and vascular myocytes where it is found preferentially sequestered to β-arrestin. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Product information
Form Liquid
Formulation 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3
Avoid freeze/thaw Yes
Unit definition One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5 at 30°C.
Activity ≥ 0.5 U/µl
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling
Data

Increasing concentration of PDE inhibitor, rolipram, was added to enzyme and the activity assayed.

© Merck KGaA, Darmstadt, Germany, 2013

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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