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EMD Millipore
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25 May 2012
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Detects and quantifies the level of the STAT1 phosphorylated at Tyr701. STAT1 is a member of STAT family of proteins that phosphorylates at Tyr701 and is essential for normal transcriptional activity of ISGF3 (interferon-stimulated gene factor 3) complex. It participates in the regulation of cytokine-signaling and cellular responses and plays an important role in growth and differentiation in a variety of cell types. This kit is designed for use with human cells and tissues.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Species reactivity | human | ||
| Sensitivity | < 0.9 Unit/ml | ||
| Assay range | 1.6-100 units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Kit contains | STAT1 (Tyr70¹) Standard, Diluents, Detection Antibody, Secondary Antibody, Sample Buffer, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of STAT1 (pTyr<sup>701</sup>). The data presented in Figure 1 show that the sensitivity of the ELISA is ~4x greater than that of immunoblotting. The bands shown in the immunoblotting data were developed using an anti STAT1 (pTyr<sup>701</sup>) antibody and chemiluminescent detection. |
