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EMD Millipore
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25 May 2012
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Detects and quantifies the level of c-Src protein that is phosphorylated at Tyr418. c-Src is the member of homologous non-receptor tyrosine kinases family which is critically involved in carcinoma cell adhesion, migration, metastasis, and the enhancement of cell survival. Although this kit is designed for use with human cell lines, platelets, and lymphocytes, it cross-reacts with mouse and rat cells.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Sensitivity | ≤ 1 Unit/ml | ||
| Assay range | 1.6-100 Units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Kit contains | Src (Tyr4¹8) Standard, Diluents, Detector Antibody, Secondary Antibody, Coated 96-Well Plate, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Canadian export regulations | Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada. | ||
| Data | |||
|---|---|---|---|
 The analytical sensitivity of this assay is <1.0 Unit/ml of Src (pTyr<sup>418</sup>). This was determined by adding two standard deviations to the mean Abs obtained when the zero standard was assayed 30. The sensitivity of this ELISA was compared to immunoblotting using known quantities of Src (pTyr<Sup>418</sup>). The data presented in Figure 1 show that the sensitivity of the ELISA is approximately the same as that of immunoblotting. The bands shown in the immunoblotting data were developed using an anti Src (pTyr<sup>418</sup>) antibody and chemiluminescent detection.  Natural Src (pTyr<sup>418</sup>) from extracts of platelets and Colo 201 cells cultured in RPMI + 10% FCS were serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the Src (pTyr<sup>418</sup>) standard curve. Parallelism demonstrated in Figure 2 indicates that the standard accurately reflects full length Src (pTyr<sup>418</sup>) content in samples. |
