569373
PhosphoDetect™ Anti-Src (pTyr4¹8) Rabbit pAb
Anti-pp60src, Phospho-Specific (Tyr4¹8)
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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
22 May 2013
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Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src.
1 T is sufficient antibody for one immunoblot using a 10 ml volume.
| Alternatives | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| Alternative products for PhosphoDetect™ Anti-Src (pTyr4¹8) Rabbit pAb | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| Accessories for PhosphoDetect™ Anti-Src (pTyr4¹8) Rabbit pAb |
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr4¹8 phosphorylation site of human Src | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.2-7.4. | ||
| Species reactivity | chicken, human, mouse | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Chicken embryo fibroblasts (CEF) expressing human wild type src | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
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 Detection of human Src, phosphorylated on Tyr418, by immunoblotting. Samples: Whole cell lysate from chick embryo fibroblasts cells untransfected (lane 1) or transfected with wild-type human Src (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Rabbit pAb (Cat. No. 569373) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence. |
