For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes a broad spectrum of tyrosine-phosphorylated proteins in crude extracts. Supplied with a mixture phosphotyrosine-containing proteins as a positive control.
| Substitutes | |
|---|---|
| 525326 | PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (P3-54) |
| Substitutes for PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (2C8) | |
| 525326 | PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (P3-54) |
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | a phosphotyrosine-containing peptide, conjugated to KLH | ||
| Clone | 2C8 | ||
| Form | Lyophilized | ||
| Formulation | 100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl positive control lyophilized from PBS, 0.1% SDS, PEG, sucrose, Malachite Green. | ||
| Species reactivity | a broad range of species | ||
| Preservative | ≤0.1% sodium azide (antibody only) | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Phosphotyrosine molecular weight markers (included) | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of phosphotyrosine-containing proteins by immunoblotting. Sample: Whole cell lysates from A431 cells treated with pervanadate (all lanes). Primary antibody: Anti-Phosphotyrosine (2C8) Mouse mAb (Cat. No. 525293) (0.5 μg/ml). Detection: chemiluminescence. |
