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525287  PhosphoDetect™ Anti-Phosphothreonine Mouse mAb (1E11)

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25 May 2012

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Product number Qty/Pk Quantity Price Delivery Date
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525287-1SET  1 set 
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Recognizes a broad range of threonine-phosphorylated proteins in crude extracts. Supplied with a positive control.
Product information
Host Mouse
Isotype IgG1κ
Immunogen a mixture of synthetic phosphopeptides containing phosphorylated threonine
Clone 1E11
Form Lyophilized
Formulation 100 µg antibody lyophilized from 2X PBS, PEG, sucrose and a control lysate lyophilized from 2X PBS, 10 mM NaF, PEG.
Species reactivity a broad range of species
Preservative ≤0.1% sodium azide (antibody and control lysate)
Applications
Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Avoid freeze/thaw Yes
Store and ship information
Storage -20°C
Ship Dry Ice Only
Standard Handling
Data

Detection of phosphothreonine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphotheonine (1E11) (Mouse) (Cat. No. 525287) (1 μg/ml). Detection: chemiluminescence *Positive control supplied with the antibody. Available separately: Anti-Phosphothreonine (14B3)(Mouse) (Cat. No. 525286), and Phosphothreonine Detection Kit (Cat. No. 525288)

© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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