For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes a broad range of serine-phosphorylated proteins in crude extracts from multiple species, requiring either proline or lysine C-terminal to the phosphoserine. Supplied with a positive control.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgM | ||
| Immunogen | a mixture of phosphopeptides containing phosphorylated serine, conjugated to KLH | ||
| Clone | 16B4 | ||
| Form | Lyophilized | ||
| Formulation | 50 µg antibody lyophilized from 2X PBS, PEG, sucrose and control lysate lyophilized from 2X PBS, PEG, sucrose, 10 mM NaF. | ||
| Species reactivity | a broad range of species | ||
| Preservative | ≤0.1% sodium azide (antibody and control lysate) | ||
| Applications |
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| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of phosphoserine-containing proteins by immunoblotting. Sample: Whole cell lysate from A431 cells treated with pervanadate (all lanes). Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282).  Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282). |
