PhosphoDetect™ Anti-IRS-1 (pTyr6¹2) Rabbit pAb
Anti-Insulin Receptor Substrate-1, Phospho-Specific (Tyr6¹2), Chico protein dIRS
For general questions please contact our Customer Service:
290 Concord Road
01821 Billerica, MA
Phone: 781 533-6000
22 May 2013
This product has been discontinued.
Recognizes the ~165-180 kDa IRS-1 protein phosphorylated at Tyr612.
|Immunogen||a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr6¹2 phosphorylation site of human IRS-1|
|Formulation||In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA (IgG, protease free) as a carrrier, pH 7.3.|
|Preservative||≤0.1% sodium azide|
|Positive control||CHO cells expressing human insulin receptor (CHO-T), subsequently transfected with human IRS-1, stimulated with insulin|
|Store and ship information|
Blue Ice Only
Detection of human IRS-1, phosphorylated at Tyr612, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with human IRS-1, left untreated (lane 1) or treated with insulin (50 nM for 5 min) (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-IRS1 (pTyr612) Rabbit pAb (Cat. No. PS1010) (0.5 µg/ml) pre-incubated without peptide (lanes 1,2), with a generic phosphotyrosine-containing peptide (lane 3), non-phosphopeptide corresponding to the immunogen (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.