PS1010
PhosphoDetect™ Anti-IRS-1 (pTyr6¹2) Rabbit pAb
Anti-Insulin Receptor Substrate-1, Phospho-Specific (Tyr6¹2), Chico protein dIRS
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
22 May 2013
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This product has been discontinued.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr6¹2 phosphorylation site of human IRS-1 | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA (IgG, protease free) as a carrrier, pH 7.3. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | CHO cells expressing human insulin receptor (CHO-T), subsequently transfected with human IRS-1, stimulated with insulin | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Irritant |
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| Data | |||
|---|---|---|---|
 Detection of human IRS-1, phosphorylated at Tyr612, by immunoblotting. Samples: Whole cell lysate from CHO-T cells transfected with human IRS-1, left untreated (lane 1) or treated with insulin (50 nM for 5 min) (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-IRS1 (pTyr612) Rabbit pAb (Cat. No. PS1010) (0.5 µg/ml) pre-incubated without peptide (lanes 1,2), with a generic phosphotyrosine-containing peptide (lane 3), non-phosphopeptide corresponding to the immunogen (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence. |
