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EMD Millipore
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25 May 2012
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Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form phosphorylated at Ser139.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹39 phosphorylation site of human H2AX | ||
| Form | Liquid | ||
| Formulation | In Tris/citrate-phosphate buffer, pH 7.0-8.0. | ||
| Species reactivity | human, mouse | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Positive control | HEK293 cell nuclear extract | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Highly Toxic |
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| Data | |||
|---|---|---|---|
 Detection of human and mouse γ-H2AX, phosphorylated at Ser<sup>139</sup> by immunoblotting. Samples: Nuclear extract (50 µg) from HEK293 cells, G361 cells, mouse wild-type, γ-H2AX embryonic fibroblasts (+/+), and mouse γ-H2AX knockout embryonic fibroblasts (-/-), all untreated (UT) or treated with neocarzinostatin (NCS) (200 ng/ml, 30 min). Primary antibody: PhosphoDetect™ Anti-γ-H2AX (pSer<sup>139</sup>) Rabbit pAb (1:10,000). Detection: chemiluminescence. |
