PhosphoDetect™ Anti-eIF-2Bε (pSer539) Rabbit pAb
Anti-EIF2B epsilon, Phospho-Specific (Ser539), Anti-Eukaryotic Initiation Factor 2B, epsilon Subunit, Phospho-Specific (Ser539)
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19 May 2013
This product has been discontinued.
Recognizes the ~82 kDa eIF-2Bε protein phosphorylated at Ser539 in CHO-T cells transfected with human insulin receptor and treated with insulin.
|Immunogen||a synthetic phosphopeptide corresponding to amino acids surrounding the Ser535 phosphorylation site of rat eIF-2B|
|Formulation||In Dulbecco's PBS (without Mg+2 and Ca+2), 50% glycerol, 1 mg/ml BSA, pH 7.3.|
|Species reactivity||human, rat|
|Preservative||≤0.1% sodium azide|
|Protect from Light||Yes|
|Positive control||Recombinant eIF-2B, CHO-T cells transfected with human insuling receptor and treated with insulin|
|Store and ship information|
Shipped with Blue Ice or with Dry Ice
Detection of eIF2Bε, phosphorylated at ser539, by immunoblotting. Samples: Recombinant eIF2Bε treated with lambda phosphatase (lane 1) or whole cell lysates from controll cells, spiked with GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-eIF2Bε (pSer539) Rabbit pAb (Cat. No. PS1017) (1:1000) preincubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or immunogen phosphopeptide (lane 5). Detection: chemiluminescence.