PS1017
PhosphoDetect™ Anti-eIF-2Bε (pSer539) Rabbit pAb
Anti-EIF2B epsilon, Phospho-Specific (Ser539), Anti-Eukaryotic Initiation Factor 2B, epsilon Subunit, Phospho-Specific (Ser539)
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
19 May 2013
loading
This product has been discontinued.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic phosphopeptide corresponding to amino acids surrounding the Ser535 phosphorylation site of rat eIF-2B | ||
| Form | Liquid | ||
| Formulation | In Dulbecco's PBS (without Mg+2 and Ca+2), 50% glycerol, 1 mg/ml BSA, pH 7.3. | ||
| Species reactivity | human, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
|
||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Positive control | Recombinant eIF-2B, CHO-T cells transfected with human insuling receptor and treated with insulin | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
||
| Data | |||
|---|---|---|---|
 Detection of eIF2Bε, phosphorylated at ser539, by immunoblotting. Samples: Recombinant eIF2Bε treated with lambda phosphatase (lane 1) or whole cell lysates from controll cells, spiked with GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-eIF2Bε (pSer539) Rabbit pAb (Cat. No. PS1017) (1:1000) preincubated without peptide (lanes 1,2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine-containing peptide (lane 4), or immunogen phosphopeptide (lane 5). Detection: chemiluminescence. |
