NB13
PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr286) (Ab-2) Mouse mAb (22B1)
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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated at Thr286. Does not cross-react with unphosphorylated α subunit of CaM kinase II or with the β subunit in either phosphorylation state.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | a synthetic phosphopeptide (MHRQEpTVDCLKKFN) corresponding to amino acids 281-294 surrounding the Thr286 phosphorylation site of mouse and rat CaM kinase II α subunit | ||
| Clone | 22B1 | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of rat Ca<sup>2+</sup>/calmodulin kinase II α-subunit, phosphorylated on Thr<sup>286</sup>, by immunoblotting. Samples: Whole tissue extract from rat brain, unphosphorylated (lane 1) and phosphorylated (lane 2). Primary antibody: PhosphoDetect™ Anti-Ca<sup>2+</sup>/Calmodulin Kinase II α-Subunit (pThr<sup>286</sup>) (Ab-2) Mouse mAb (22B1) (Cat. No. NB13) (1:2000). Detection: chemiluminescence. |
