CBA004
PhosphoDetect™ Akt (pThr308) ELISA Kit
Protein Kinase B, Phospho-Specific (Thr308) ELISA Kit
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Detects Akt when phosphorylated on Thr308. Akt is activated by a variety of stimuli including insulin through phosphorylation on Thr308 and Ser473. Once phosphorylated Akt migrates to the nucleus where it is involved in a variety of cellular processes such as the transduction of signals that prevent apoptosis. Although this kit was developed for human samples, it has been found to cross react with mouse and rat.
| Alternatives | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| Alternative products for PhosphoDetect™ Akt (pThr308) ELISA Kit | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| Accessories for PhosphoDetect™ Akt (pThr308) ELISA Kit |
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Avoid freeze/thaw | Yes | ||
| Sensitivity | ≤0.8 units/ml | ||
| Assay range | 1.6-100 units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates | ||
| Positive control | Akt (pThr308) | ||
| Kit contains | Coated 96-Well Plate, Akt Phospho-Thr308 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
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 The data presented in figure 1 shows that the sensitivity of the ELISA is approximately the same as that of immunoblotting. The bands shown in the immunoblot were developed using Anti-Akt, Phospho-Specific (Thr<sup>308</sup>), (Cat. No. 124001), an alkaline phosphatase conjugated anti-rabbit IgG analyzed by chemiluminescent substrate, and autoradiography. |
