CBA005
PhosphoDetect™ Akt (pSer473) ELISA Kit
Protein Kinase B, Phospho-Specific (Ser473) ELISA Kit
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Detects Akt1 when phosphorylated on Ser473. Does not detect phosphorylated Akt3. Cross-reactivity with phosphorylated Akt2 has not been thoroughly evaluated. Akt is activated by a variety of stimuli including insulin through phosphorylation on Thr308 and Ser473. Once phosphorylated Akt migrates to the nucleus where it is involved in a variety of cellular processes such as the transduction of signals that prevent apoptosis. Although this kit was developed for human samples, it has been found to cross react with mouse and rat.
| Alternatives | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| Alternative products for PhosphoDetect™ Akt (pSer473) ELISA Kit | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| Accessories for PhosphoDetect™ Akt (pSer473) ELISA Kit |
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit is defined as the amount of Akt (pSer473) derived from 100 pg of Akt, which was phosphorylated by MAPKAP2 and PDK1. | ||
| Sensitivity | ≤0.8 units/ml | ||
| Assay range | 1.6-100 units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates | ||
| Positive control | Akt (pSer473) | ||
| Kit contains | Coated 96-Well Plate, Akt Phospho-Ser473 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
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| S Phrase |
S: 26-36/37-45 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 21/22-36/38 Harmful in contact with skin and if swallowed. Irritating to eyes and skin. |
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