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70072  pET-32 Xa/LIC Vector Kit

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19 June 2013

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70072-3  20 rxn 
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The pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...




The pET System allows for expression of target genes under control of the T7 promoter and is covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs. Commercial entities need to obtain a license from Brookhaven National Labs prior to use of this Product. Ccommercial use of this Product also requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
Product information
Avoid freeze/thaw Yes
Components
1 µgXa/LIC Vector
10 µlXa/LIC Control Insert
25 UT4 DNA Polymerase, LIC-qualified
50 µl10X T4 DNA Polymerase Buffer
100 µl100 mM DTT
50 µl25 mM EDTA
40 µl25 mM dGTP
1.5 mlNuclease-free Water
22 × 50 µlNovaBlue GigaSingles™ Competent Cells
0.2 mlBL21(DE3) Competent Cells
0.2 mlBL21(DE3)pLysS Competent Cells
5 × 2 mlSOC Medium
10 µlTest Plasmid
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2013

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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