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EMD Millipore
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25 May 2012
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Recombinant, human PERK consisting of amino acids 563-1115 fused at the N-terminus to GST and expressed in E. coli. PERK phosphorylates the α-subunit of eukaryotic translation initiation factor 2 (EIF2α), which leads to its inactivation and reduces translation initiation. The active kinase is useful for the study of PERK regulation and for inhibitor screening.
Note: the calculated MW is 88,000, but the protein runs at ~115,000 on SDS-PAGE.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 50 mM Tris-HCl, 50 mM NaCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5. | ||
| Molar mass | 115000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥26 nmol/min/mg protein | ||
| Unit definition | Kinase activity is measured as the molar amount of phosphate incorporated into SMAD3 at 30°C using a final concentration of 50 µM ATP. | ||
| Purity | ≥90% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 The activity of PERK was measured as outlined in the Recommended Reaction Conditions. |
