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Pellet Paint® Co-Precipitant

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Pellet Paint^® Co-Precipitant* is a visible dye-labeled carrier formulated specifically for use in alcolhol precipitation of nucleic acids. The five-minute precipitation protocol requires no low temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from solutions as dilute as 2 ng/ml, and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps, preventing losses during handling. Pellet Paint is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes.

*Patent pending

Pellet Paint^® NF Co-Precipitant* is a non-fluorescent dye labeled carrier compatible with fluorescent sequencing. It can increase precipitation speed and efficiency, lower centrifugation times, and help rapidly clean up unincorporated BigDye™ terminators¹. Since nucleic acid pellets with Pellet Paint NF Co-Precipitant are so visible, small pellets are unlikely to be lost and pellet dissolution is clearly visible.
Pellet Paint NF Co-Precipitant is fully compatible with the ABI PRISM™ DNA BigDye Terminator Cycle Sequencing Ready Reaction kit. To avoid extra sample handling, Pellet Paint NF Co-Precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer prior to the cycle sequencing reaction. Pellet Paint NF Co-Precipitant has no detectable effect on the sequencing reaction or sequencing accuracy.

¹BigDye and PRISM are trademarks of Applied Biosystems.

Features:

Convenient	No low temperature incubation; incubate at room temperature No prolonged centrifugation
Compatible	Compatible with numerous downstream applications Compatible with the use of sodium acetate, sodium chloride, and ammonium acetate salts Compatible with ethanol and isopropanol precipitations
Pure	Preparation is free of contaminating nucleic acids and nucleolytic enzymes
Reliable	Pellet is easily visualized through precipitation, washes and solubilization, minimizing losses during handling (i.e. inadvertent discarding of pellet) Improves reproducibility and sensitivity in certain applications (e.g. PCR)
Flexible	Suitable for DNA and RNA precipitation Works even with minute amounts (< 2 ng/ml) of nucleic acid
Efficient	Quick, 7 minute protocol Small single and double stranded molecules and unincorporated mononucleotides are efficiently removed
Easy to Use	Forms tight pellets which do not spread along tube walls, so exact location of pellet is always known

Features:

Expedient	Helps precipitate nucleic acids quickly and quantitatively Speeds techniques like BigDye terminator removal
Convenient	10 minute room temperature incubation Shortened centrifugation time (even with small quantities of nucleic acid)
Compatible	Compatible with fluorescent sequencing Compatible with sodium acetate, sodium chloride, and ammonium acetate salts Compatible with ethanol and isopropanol precipitations
Pure	Free of contaminating nucleic acids and nucleolytic enzymes
Flexible	Suitable for DNA and RNA precipitation Even works with minute quantities of nucleic acid (< 2 ng/ml)
Saves Time and Money	Much cheaper than spin filter dye terminator cleanup Prevents lost experiments and time

Product

125 rxn Pellet Paint® Co-Precipitant
Each kit contains:

250 µl Pellet Paint Co-Precipitant
3 M Sodium Acetate, pH 5

Product

125 rxn Pellet Paint® NF Co-Precipitant
Each kit contains:

250 µl Pellet Paint NF Co-Precipitant
3 M Sodium Acetate, pH 5.S

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