521270
PDK1, His•Tag®, Human, Recombinant, S. frugiperda
3-Phosphoinositide-Dependent Protein Kinase-1
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Full length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5. | ||
| Molar mass | 66867 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥80 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5. | ||
| Purity | ≥90% by SDS-PAGE | ||
| EC number | 2.7.10 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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