p70S6K, His•Tag®, Human, Recombinant, S. frugiperda
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01821 Billerica, MA
Phone: 781 533-6000
22 May 2013
This product has been discontinued.
Full-length, recombinant, human p70S6 Kinase (p70S6K) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. p70S6K is a ubiquitously expressed serine/threonine protein kinase that can be activated by serum stimulation and inhibited by wortmannin and rapamycin. Activation of p70S6K requires phosphoinositide 3-kinase (PI-3K)-dependent and mTOR-dependent signals. The catalytically active enzyme is useful for inhibitor and activator assays.
|Formulation||In 150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol, pH 7.5.|
|Specific activity||≥78 U/mg protein|
|Unit definition||One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to p70S6K synthetic substrate, CKRRRLASLR, per min at 30°C, pH 7.2.|
|Purity||≥80% by SDS-PAGE|
|Store and ship information|
Dry Ice Only