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QIA07  p53 ELISA Kit (Pantropic)

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25 May 2012

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Suitable for the in vitro quantitation of mutant and wild-type p53 protein. The human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer with the majority of mutations being amino acid substitutions. p53 is targeted for inactivation by transforming viral oncoproteins. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage.
Product information
Format 96-well plate
Form 96 Tests
Detection method Colorimetric
Species reactivity human
Do not freeze Yes
Specificity Mutant and wild-type p53
Sensitivity 10 pg/ml
Assay range 93.75 - 1500 pg/ml
Assay time Overnight
Sample type Cell extracts, tissue extracts, conditioned media, sera, urine
Kit contains Anti-Panp53 Coated 96-Well Plate, 2 vials of Lyophilized Standards, Reporter Antibody Peroxidase Conjugate, Normal Mouse Serum (Cat. No. NS03L), Antigen Extraction Agent, Peroxidase Substrate, Diluents, Buffers, Stop Solution, and a user protocol.
Store and ship information

Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Storage +2°C to +8°C
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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